Source Name	Characteristics[sample_name]	Characteristics[organism]	Characteristics[taxonomy_id]	Characteristics[strain]	Characteristics[cell_line]	Characteristics[cell_type]	Characteristics[genetic_modification]	Comment[BioSample]	Comment[sample_title]	Comment[description]	Protocol REF	Protocol REF	Protocol REF	Extract Name	Material Type	Protocol REF	Labeled Extract Name	Label	Protocol REF	Assay Name	Technology Type	Array Design REF	Protocol REF	Array Data File	Protocol REF	Derived Array Data Matrix File	Factor Value[genetic modification]
IGFBP2 knockdown U251 subline	IGFBP2 knockdown U251 subline	Homo sapiens	9606	IGFBP2 knockdown subline	U251	Human glioblastoma	gene_knock_in	SAMD00160559	IGFBP2 knockdown U251 subline	The knockdown vector was constructed by using a shRNA expression retroviral vector pSINsi-hU6 (TAKARA Bio, Shiga, Japan). The most efficient target sequence for RNA interference was selected among six. U251 cells were exposed to the virus pools produced by transfection of the vector into Amphopack293 packaging cells. The transfected cells were subcultured at an appropriate density in fresh DMEM containing 0.5 mg/ml. The G418-resistant cell pools were readily established within 2 weeks.	P-GEAD-100	P-GEAD-101	P-GEAD-106	IGFBP2 knockdown U251 subline	total RNA	P-GEAD-102	IGFBP2 knockdown U251 subline	biotin	P-GEAD-103	IGFBP2 knockdown U251 subline	array assay	A-AFFY-113	P-GEAD-104	KD_U251_t.txt	P-GEAD-105	U251_t.txt	IGFBP2 knockdown
Control U251 subline	Control U251 subline	Homo sapiens	9606	control subline (infected of the retrovirus expressing short hairpin RNA containing scrambled sequence)	U251	Human glioblastoma	gene_knock_in	SAMD00160560	Control U251 subline	The knockdown vector was constructed by using a shRNA expression retroviral vector pSINsi-hU6 (TAKARA Bio, Shiga, Japan). The most efficient target sequence for RNA interference was selected among six. U251 cells were exposed to the virus pools produced by transfection of the vector into Amphopack293 packaging cells. The transfected cells were subcultured at an appropriate density in fresh DMEM containing 0.5 mg/ml. The G418-resistant cell pools were readily established within 2 weeks.	P-GEAD-100	P-GEAD-101	P-GEAD-106	Control U251 subline	total RNA	P-GEAD-102	Control U251 subline	biotin	P-GEAD-103	Control U251 subline	array assay	A-AFFY-113	P-GEAD-104	Control_U251_t.txt	P-GEAD-105	U251_t.txt	control