Comment[GEAAccession]	E-GEAD-299
MAGE-TAB Version	1.1
Investigation Title	Comparison of gene expression profiles between human AML stem cells and non-stem fractions
Experiment Description	To understand key molecules regulating stemness of human leukemia, we compared gene expressions between CD34+CD38- stem cells and CD34+CD38+ non-stem fractions.
Experimental Design	cell type comparison design
Experimental Factor Name	cell type
Experimental Factor Type	cell type
Person Last Name	Ishikawa
Person First Name	Fumihiko
Person Affiliation	Research Unit for Human Disease Model,RCAI,The Institute of Physical and Chemical Research (RIKEN)
Person Roles	submitter
Public Release Date	2019-02-01
PubMed ID	20371479
Protocol Name	P-GEAD-113	P-GEAD-114	P-GEAD-115	P-GEAD-116	P-GEAD-117	P-GEAD-118
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid labeling protocol	nucleic acid hybridization to array protocol	array scanning and feature extraction protocol	normalization data transformation protocol
Protocol Description	Not provided	Performed using RNAeasy (QIAGEN)	Performed using Two-cycle target labeling kit (Affymetrix, Inc.)	http://www.affymetrix.com/support/technical/manual/expression_manual.affx	By using GCOS software (Affymetrix, Inc.) By using GeneChip Scanner (Affymetrix, Inc.)	Data normalization was performed gc-RMA algorithm using GeneSpring package (Agilent)
SDRF File	E-GEAD-299.sdrf.txt
Comment[Number of channel]	single-channel
Comment[CIBEX Submitter]	Fumihiko Ishikawa
Comment[Array Design REF]	A-AFFY-113
Comment[AEExperimentType]	transcription profiling by array
Comment[SecondaryAccession]	CBX21
Comment[BioProject]	PRJDB7985
Comment[CIBEX Accept Date]	2007-04-19
Comment[CIBEX Public Release Date]	2007-12-27
Comment[Last Update Date]	2019-08-14