Comment[GEAAccession]	E-GEAD-300
MAGE-TAB Version	1.1
Investigation Title	Molecular biological validation of the effect of Japanese traditional multi-herbal medicine on the senescence
Experiment Description	The Japanese traditional medicine (ie. Kampo medicine) consists of several herbs in a single formula. However, the mechanism of action from the viewpoint of elucidation of molecular biology is not known at all. Here, the RNA-seq method was applied to characterize the effect of Kampo medicine in klotho mouse, a model mouse for aging.
Experimental Design	compound treatment design
Experimental Factor Name	drug treatment
Experimental Factor Type	drug treatment
Person Last Name	Oshima	Okada
Person First Name	Kenshiro	Norihiro
Person Affiliation	Coelacanth Institute, Foundation for Advancement of International Science	
Person Roles	submitter	submitter
Public Release Date	2023-02-01
Protocol Name	P-GEAD-119	P-GEAD-120	P-GEAD-121	P-GEAD-122	P-GEAD-123
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	Liver sample from male alpha-Klotho(kl-/-/Jcl) mouse with/without JTT and male WT(C57BL/JJcl) mouse with/without JTT	Pure Link RNA Mini Kit	TruSeq Stranded mRNA Sample Prep Kit	Paired-end (150 base pairs X 2) sequencing	TMM normalization
SDRF File	E-GEAD-300.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB7898
Comment[Last Update Date]	2023-02-01