Comment[GEAAccession]	E-GEAD-303
MAGE-TAB Version	1.1
Investigation Title	Mastication affects transcriptomes of mouse microglia
Experiment Description	We investigated whether mastication affects microglia by comparing microglia transcriptomes from hard-diet-fed mice with those from soft-diet-fed mice. We kept twelve male mice of 10 weeks old by feeding either a hard or soft diet for 2, 4 or 8 months. After each period, we removed the whole brains from four mice, each two of which were fed with either a hard or soft diet, and isolated microglia from each brain. The total RNA extracted from each brain's microglia was subjected to DNA microarray analysis.
Experimental Design	stimulus or stress design	time series design
Experimental Factor Name	diet/month
Experimental Factor Type	diet/month
Person Last Name	Nashimoto
Person First Name	Masayuki
Person Affiliation	Niigata University of Pharmacy and Applied Life Sciences
Person Roles	submitter
Public Release Date	2019-03-13
Protocol Name	P-GEAD-137	P-GEAD-138	P-GEAD-139	P-GEAD-140	P-GEAD-141	P-GEAD-142
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid labeling protocol	nucleic acid hybridization to array protocol	array scanning and feature extraction protocol	normalization data transformation protocol
Protocol Description	Twelve C3H/HeNJcl male mice of 10 weeks old were purchased from Charles River Laboratories (Kanagawa, Japan).  After a one-week acclimation-breeding period, a half of them were fed with a pelleted (hard) diet (CE-2, FEED ONE, Kanagawa, Japan) and another half were with a powdered (soft) diet (CE-2) for 2, 4 or 8 months. These diets contained the same ingredients. After 2, 4 or 8 months, four mice, each two of which were fed with either a hard or soft diet, were euthanized by cervical dislocation, and the whole brains were removed. Microglia were isolated from each brain according to the established method.	Each total RNA from microglia of the 2-month-fed mice was extracted with RNAiso Plus (Takara Bio, Otsu, Japan). Each total RNA from microglia of the 4-month- and 8-month-fed mice was extracted with an miRNeasy Micro Kit (Qiagen).	According to Agilent instructions.	According to Agilent instructions.	According to Agilent instructions.	According to Agilent instructions.
SDRF File	E-GEAD-303.sdrf.txt
Comment[Number of channel]	single-channel
Comment[Array Design REF]	A-GEOD-21810
Comment[AEExperimentType]	transcription profiling by array
Comment[BioProject]	PRJDB7954
Comment[Last Update Date]	2019-08-14