Comment[GEAAccession]	E-GEAD-321
MAGE-TAB Version	1.1
Investigation Title	Expression profiles of HB1119 and 293T cell lines
Experiment Description	HB1119 cells and 293T cells were analysed by mRNA-seq. HB1119 cells are human leukemia cells that endogenously express the MLL-ENL fusion protein. 293T cells express wildtype MLL and ENL.
Experimental Design	cell type comparison design
Experimental Factor Name	cell
Experimental Factor Type	cell
Person Last Name	Kanai	Yokoyama
Person First Name	Akinori	Akihiko
Person Affiliation	Tsuruoka Metabolomics Laboratory, National Cancer Center	
Person Roles	submitter	submitter
Public Release Date	2020-12-10
PubMed ID	28394257
Protocol Name	P-GEAD-241	P-GEAD-242	P-GEAD-243	P-GEAD-244	P-GEAD-245
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	HEK293T cells are culture in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and penicillin-streptomycin (PS).HB1119 cells were cultured in RPMI 1640 medium supplemented with 10% FBS and PS. Cell culture was performed in 5% CO2 37 degree C.	Total RNA was prepared using the RNeasy kit. Quality Check of RNA was performed using Agilent Bioanalyzer RNA 6000 nano kit.	The libralies were constructed using a SureSelect Strand Specific RNA Library Prep Kit (Agilent) following the manufacturer's instructions.	The libraries of HB1119 were sequenced for 36 cycles on an Illumina GAIIx sequencer with 36-bp single-end reads. The libraries of 293T were sequenced for 51 cycles on an Illumina HiSeq 2500 sequencer with 51-bp single-end reads.	Sequenced reads were mapped to human genome assembly hg19 using CASAVA 1.8.2 and gene expression was normalized as reads per kilo base of exon per million mapped (RPKM)
SDRF File	E-GEAD-321.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB4966
Comment[Last Update Date]	2020-12-10