Comment[GEAAccession] E-GEAD-332 MAGE-TAB Version 1.1 Investigation Title The Y4RNAfr affects transcriptomes Experiment Description We investigated how the Y4RNAfr affects the transcriptomes of human cell lines, HL60, RPMI-8226, and HEK293. Experimental Design compound treatment design Experimental Factor Name cell_line] Factor Value[RNA_treatment Experimental Factor Type cell_line] Factor Value[RNA_treatment Person Last Name Nashimoto Person First Name Masayuki Person Affiliation Niigata University of Pharmacy and Applied Life Sciences Person Roles submitter Public Release Date 2019-11-19 Protocol Name P-GEAD-303 P-GEAD-304 P-GEAD-305 P-GEAD-306 P-GEAD-307 P-GEAD-308 P-GEAD-309 Protocol Type sample collection protocol nucleic acid extraction protocol nucleic acid labeling protocol nucleic acid hybridization to array protocol array scanning and feature extraction protocol normalization data transformation protocol treatment protocol Protocol Description HL60 and RPMI-8226 cells were cultured in RPMI-1640 supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin at 37 degrees C in 5% CO2 humidified incubator. HEK293 cells were cultured in DME media likewise. Each total RNA was extracted with RNAiso Plus. Agilent standard protocol. Agilent standard protocol. Agilent standard protocol. Agilent standard protocol. HL60 and RPMI-8226 cells and HEK293 cells were cultured in media without and with 1 microM of the naked Y4RNAfr. As controls, the cells were also cultured in the presence of 1 microM of naked CR2 or CR3 for HL60 cells, 1 microM of naked CR4 for RPMI-8226 cells, and 1 microM of naked CR5 for HEK293 cells. SDRF File E-GEAD-332.sdrf.txt Comment[Number of channel] single-channel Comment[Array Design REF] A-MEXP-2320 Comment[AEExperimentType] transcription profiling by array Comment[BioProject] PRJDB8981 Comment[Last Update Date] 2019-11-19