Comment[GEAAccession]	E-GEAD-334
MAGE-TAB Version	1.1
Investigation Title	transcriptome analysis of mouse cardiac fibroblast related to Tcf21
Experiment Description	"\"\"\"\"\"\"We isolated mouse cardiac fibroblast from E18.5 mouse heart (Tcf21 wild type and KO). These cells were cultured and passaged. Within passage number 3, total RNA was obtained from fibroblasts with using the PureLink RNA Mini Kit.

Each group (WT vs KO) has three sample obtained, and each sample was obtained from different animal.\"\"\"\"\"\""
Experimental Design	genotype design
Experimental Factor Name	genetic_modification
Experimental Factor Type	genetic_modification
Person Last Name	Baba	Maezawa
Person First Name	Yusuke	Yoshiro
Person Affiliation	Department of Endocrinology, Hematology and Gerontology, Chiba University Graduate School of Medicine	
Person Roles	submitter	submitter
Public Release Date	2023-11-30
Protocol Name	P-GEAD-316	P-GEAD-317	P-GEAD-318	P-GEAD-319	P-GEAD-320
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	Cardiac fibroblasts were obtained from E18.5 mouse heart.	Total RNA was extracted using the PureLink RNA Mini Kit (Invitrogen, Carlsbad, USA)	cDNA libraries were generated using a NEBNext Ultra RNA Library Prep Kit (New England BioLabs, Beverly, USA).	Sequencing was performed using HiSeq 1500 (Illumina) with a single-read sequencing length of 60bp.	Quality control was performed using FastQC. Trimmomatic v0.36 was used to filter low quality reads of NGS data.
SDRF File	E-GEAD-334.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB9053
Comment[Last Update Date]	2023-11-30