Comment[GEAAccession]	E-GEAD-342
MAGE-TAB Version	1.1
Investigation Title	RNA-seq analysis in cells treated with low concentration of bortezomib
Experiment Description	RNA-seq analysis of U2OS cells treated with 10 nM bortezomib for 0 hr, 30 hr, 60 hr, and 120 hr to identify cellular response to modest and long-term proteasome inhibition. Culture medium containing bortezomib was replaced every 12 hr. Total RNA was extracted from U2OS cells using High Pure RNA isolation kit, and RNA-Seq librarlies were obtained by TruSeq library preparation kit. RNA-Seq libraries were sequenced using HiSeq 4000 sequencer, and sequence reads were mapped to the human genome (hg19) and processed by Tophat and Cufflinks.
Experimental Design	compound treatment design	time series design
Experimental Factor Name	treatment
Experimental Factor Type	treatment
Person Last Name	hamazaki
Person First Name	jun
Person Affiliation	university of tokyo
Person Roles	submitter
Public Release Date	2020-07-02
Protocol Name	P-GEAD-359	P-GEAD-360	P-GEAD-361	P-GEAD-362	P-GEAD-363
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	Total RNA from U2OS cells treated with 10 nM bortezomib was  collected.	Total RNA was extracted from U2OS cells using a High Pure RNA isolation kit (Roche).	illumina TruSeq RNA Library Prep Kit v2	illumine HS4000 PE100	Expression are evaluated by FPKM analysed by RSEM.
SDRF File	E-GEAD-342.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB8235
Comment[Last Update Date]	2020-07-02