Comment[GEAAccession]	E-GEAD-343
MAGE-TAB Version	1.1
Investigation Title	Aspirin broadly upregulates glutaminolysis-related genes in PIK3CA CRC cells
Experiment Description	RNA-seq data of HCT-15 cells treated with DMSO or 2 mM aspirin for 24 hr (n=2). To identify differentially regulated pathways affected by aspirin, we performed RNA sequencing analysis of HCT-15 cells treated with DMSO or aspirin, and gene set enrichment analysis (GSEA). Aspirin can behave to imitate glutamine depletion and transcriptionally upregulate glutaminolysis-related genes in PIK3CA-mutated cells.
Experimental Design	compound treatment design	translational bias design	replicate design
Experimental Factor Name	treatment
Experimental Factor Type	treatment
Person Last Name	Boku	Hirota
Person First Name	Shogen	Kiichi
Person Affiliation	Department of Molecular-Targeting Cancer Prevention, Kyoto Prefectural University of Medicine	
Person Roles	submitter	submitter
Public Release Date	2020-05-08
Protocol Name	P-GEAD-364	P-GEAD-365	P-GEAD-366	P-GEAD-367	P-GEAD-368
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	Total RNA was extracted from cells using RNeasy Mini Kit (Qiagen) and processed using TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA, USA)	Total RNA was extracted from cells using RNeasy Mini Kit (Qiagen) and processed using TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA, USA)	RNA libres were then constructed using TruSeq Stranded mRNA Library Preparation Kit (Illumina,ari San Diego, CA, USA).	RNA libres were at 100 bp paired-ends on an Illumina NovaSeq6000 platform.	The quantified transcript reads were filtered with 0.5 counts per million (CPM) in at least one sample and transformed, using EdgeR: log2(CPM+c), with pseudocount c =4.
SDRF File	E-GEAD-343.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB9390
Comment[Last Update Date]	2020-05-08