Comment[GEAAccession]	E-GEAD-355
MAGE-TAB Version	1.1
Investigation Title	Transcriptomic data of Brown plant hopper, Nilaparvata lugens imidacloprid, susceptible or resistant strains
Experiment Description	Transcriptomic data of of Brown plant hopper, Nilaparvata lugens imidacloprid, susceptible or resistant strains were used for the identification of genes involved in  imidacloprid resistance. These transcriptomic data was calculated by RSEM bundled with Trinity r20140717.
Experimental Design	case control design
Experimental Factor Name	strain
Experimental Factor Type	strain
Person Last Name	Yokoi
Person First Name	Kakeru
Person Affiliation	Division of Applied Genetics/Insect Genome Research and Engineering Unit, Institute of Agrobiological Sciences, The National Agriculture and Food Research Organization
Person Roles	submitter
Public Release Date	2020-12-10
Protocol Name	P-GEAD-427	P-GEAD-428	P-GEAD-429	P-GEAD-430	P-GEAD-431
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	Samples were collected in paddy field of several area showing in the corresponding Biosample metadata.	Total RNA was extracted from mutiple whole animals by RNeasy Plus Mini Kit (Qiagen)	A total of 2 microgram of RNA was used for constructing paired-end libraries (Truseq library) following the manufacturer's instructions.	The libraliy were sequenced by HIseq2000 with 100-bp paired-end reads.	Illumina adapter sequences were trimed and 100 > bp sequemces in law fastq data were removed by the Trimmomatic version 0.36. The sequencial quality control were performed by FastQC Version 0.11.6. Using the sequences and TSA contig (Accession no. IACV01000001-IACV01132112), abunce related data of each contig was calculated by RSEM bundeled with Trinity r20140717.
SDRF File	E-GEAD-355.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB6524
Comment[Last Update Date]	2020-12-10