Comment[GEAAccession]	E-GEAD-360
MAGE-TAB Version	1.1
Investigation Title	Expression profiles of murine myeloid progenitors immortalized by various oncogenes
Experiment Description	Murine myeloid progenitors were harvested from C57Bl6/J mice and retrovirally transduced with FLAG-tagged MLL-AF10, HOXA9, or MYC. The cells were cultured in a semi-solid media containing SCF, IL3 and GM-CSF. RNAs prepared from 3 each cell lines were subjected to mRNA-seqencing.
Experimental Design	cell type comparison design
Experimental Factor Name	phenotype
Experimental Factor Type	phenotype
Person Last Name	Kanai	Yokoyama
Person First Name	Akinori	Akihiko
Person Affiliation	Tsuruoka Metabolomics Laboratory, National Cancer Center	
Person Roles	submitter	submitter
Public Release Date	2021-07-28
Protocol Name	P-GEAD-452	P-GEAD-453	P-GEAD-454	P-GEAD-455	P-GEAD-456
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	The myeloid progenitor cells transduced with various oncogenes were cultured in a methylcellulose medium (Iscove's Modified Dulbecco's Medium, 20% FBS, 1.6% methylcellulose, and 100 microM beta-mercaptoethanol) containing murine stem cell factors, interleukin-3, and granulocyte-macrophage colony-stimulating factor (10 ng ml-1 of each).	Total RNA was prepared using the RNeasy kit	The libralies were constructed using a SureSelect Strand Specific RNA Library Prep Kit (Agilent) following the manufacturer's instructions.	The libraries were sequenced for 51 cycles on an Illumina HiSeq 2500 sequencer with 51-bp single-end reads.	Sequenced reads were mapped to mouse genome assembly mm9 using tophat 2.0.14 and the read counts were normalized with cufflinks 2.2.1.
SDRF File	E-GEAD-360.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB9727
Comment[Last Update Date]	2021-07-28