Comment[GEAAccession]	E-GEAD-363
MAGE-TAB Version	1.1
Investigation Title	Transcriptome dataset of human corneal endothelium
Experiment Description	A transcriptome dataset from ribosomal RNA-depleted total RNA of corneal endothelium from the eyes derived from seven Caucasians without ocular diseases. Cornea endothelium is one of the five different layers of cornea. As corneal endothelium has limited proliferative capacity, damage to corneal endothelium by multiple pathological conditions, such as Fuchs endothelial corneal dystrophy (FECD; OMIM ID: 204870), invasive cataract surgery, glaucoma surgery, and endotheliitis, induce loss of corneal transparency resulting in severe visual disturbance. Therefore, the dataset should be useful for investigating the function/dysfunction of cornea as well as for the extended transcriptome analyses integrated with the data of non-coding RNAs.
Experimental Design	quality control testing design	reference design
Experimental Factor Name	donor
Experimental Factor Type	donor
Person Last Name	Nakano	Tokuda
Person First Name	Masakazu	Yuichi
Person Affiliation	Department of Genomic Medical Sciences, Kyoto Prefectural University of Medicine	
Person Roles	submitter	submitter
Public Release Date	2020-12-10
Protocol Name	P-GEAD-468	P-GEAD-469	P-GEAD-470	P-GEAD-471	P-GEAD-472
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	QIAzol lysis reagent	RNeasy Mini Kit	SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian	TruSeq PE Cluster Kit v3 and TruSeq SBS Kit v3	Tophat2 and Cufflinks
SDRF File	E-GEAD-363.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB9914
Comment[Last Update Date]	2020-12-10