Comment[GEAAccession]	E-GEAD-373
MAGE-TAB Version	1.1
Investigation Title	Transcription profiling of enteroids exposed to the luminal or basolateral deoxynivalenol.
Experiment Description	To compare the effects of deoxynivalenol on intestinal epithelial by its route of exposure, we perfomed RNA sequencing of enteroids exposed to the luminal or basolateral deoxynivalenol.
Experimental Design	observational design
Experimental Factor Name	treatment
Experimental Factor Type	treatment
Person Last Name	Hanyu	Hattori
Person First Name	Hikaru	Kazuo
Person Affiliation	Tokyo University of Agriculture	
Person Roles	submitter	submitter
Public Release Date	2020-09-29
Protocol Name	P-GEAD-523	P-GEAD-524	P-GEAD-525	P-GEAD-526	P-GEAD-527
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	Enteroids were exposed to the luminal or basolateral deoxynivalenol.	Total RNA was extracted from enteroids by using ISOGEN II, according to manufactures protocol.	The RNA library was prepared by TruSeq Stranded mRNA Library Prep. Kit, according to manufactures protocol.	The obtained cDNA pool was subjected to high-throughput sequencing using the NovaSeq 6000 system.	Known genes and transcripts are assembled with StringTie based on reference genome model. After assembly, the abundance of gene/transcript is calculated in the read count and normalized value as FPKM for a sample.
SDRF File	E-GEAD-373.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB10051
Comment[Last Update Date]	2020-09-29