Comment[GEAAccession]	E-GEAD-375
MAGE-TAB Version	1.1
Investigation Title	Strict recruitment of an RNA-dependent RNA polymerase in plant siRNA amplification
Experiment Description	To characterize the distribution of secondary siRNAs produced from Luc-gf698, small RNA sequencing (sRNA-seq) was used. We mixed Luc-gf698 and YG1 extracts with AGO1 alone, and with 21- or 22-nt siR-gf698-AGO1 in the presence or absence of SDE5 (AGO1 alone, AGO1+SDE5, 21-nt siRNA-AGO1, 21-nt siRNA-AGO1+SDE5, 22-nt siRNA-AGO1 and 22-nt siRNA-AGO1+SDE5), extracted RNA from each reaction mixture, and carried out sRNA-seq analyses.
Experimental Design	cell component comparison design
Experimental Factor Name	siRNA
Experimental Factor Type	siRNA
Person Last Name	Han	Yoshikawa
Person First Name	Yong-Woon	Manabu
Person Affiliation	Laboratory for Integrative genomics, Center for Integrative Medical Sciences, RIKEN	
Person Roles	submitter	submitter
Public Release Date	2020-12-29
Protocol Name	P-GEAD-534	P-GEAD-535	P-GEAD-536	P-GEAD-537	P-GEAD-538
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	Cell extract of Arabidopsis thaliana cell line, YG1	TRIzol LS	TruSeq Small RNA Library Preparation Kits (Illumina)	HiSeq2500 (Illumina)	cutadapt 2.7
SDRF File	E-GEAD-375.sdrf.txt
Comment[AEExperimentType]	microRNA profiling by high throughput sequencing
Comment[BioProject]	PRJDB10061
Comment[Last Update Date]	2021-08-03