Comment[GEAAccession]	E-GEAD-378
MAGE-TAB Version	1.1
Investigation Title	The Polycomb group protein Ring1 regulates dorsoventral patterning of the mouse telencephalon
Experiment Description	To examine the role of Polycomb group proteins in dorsoventral patterning, we performed CUT&Tag analysis for H3K27me3 and Ring1B using CD133-high cells from dorsal-midline, dorsal or ventral regions in the telencephalon.
Experimental Design	development or differentiation design	binding site identification design
Experimental Factor Name	antibody
Experimental Factor Type	antibody
Person Last Name	Gotoh	Kishi
Person First Name	Yukiko	Yusuke
Person Affiliation	Laboratory of Molecular Biology  Graduate School of Pharmaceutical Sciences, The University of Tokyo	
Person Roles	submitter	submitter
Public Release Date	2020-09-02
Protocol Name	P-GEAD-550	P-GEAD-551	P-GEAD-552	P-GEAD-553	P-GEAD-554
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	CD133-high cells were sorted from dorsal-midline, dorsal or ventral regions of the mouse telencephalon at E11.	CUT&Tag.	PCR amplification.	Paired end sequence.	H3K27me3 signals were normalized with human spike-in genome. Ring1B signals were normalized as CPM (count per million fragments).
SDRF File	E-GEAD-378.sdrf.txt
Comment[AEExperimentType]	ChIP-seq
Comment[BioProject]	PRJDB8285
Comment[Last Update Date]	2020-09-02