Comment[GEAAccession]	E-GEAD-379
MAGE-TAB Version	1.1
Investigation Title	mRNAseq in control and Nes:Cre_Ddx20 cKO
Experiment Description	We sequenced mRNA from mouse E14.5 embryonic spinal cords to compare gene expression level and alternative splicing events between 2 control and 2 Ddx20 cKO.
Experimental Design	development or differentiation design	genetic modification design	replicate design
Experimental Factor Name	genetic_modification
Experimental Factor Type	genetic_modification
Person Last Name	Bizen	Takebayashi
Person First Name	Norihisa	Hirohide
Person Affiliation	Division of Neurobiology and Anatomy, Graduate School of Medical and Dental Sciences, Niigata University	
Person Roles	submitter	submitter
Public Release Date	2021-12-14
Protocol Name	P-GEAD-555	P-GEAD-556	P-GEAD-557	P-GEAD-558	P-GEAD-559
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	independent two replicates of embryonic day 14.5 mouse spinal cords from control and Qk conditional knockouts.	The RNA was extracted using miRNeasy Mini Kit.	Illumina TruSeq RNA Sample Prep Kit v2	The libraries were sequenced by Illunima Hiseq 4000	Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated. In short, exons from all isoforms of a gene were merged to create one meta-transcript. The number of reads falling in the exons of this meta-transcript were counted and normalized by the size of the meta-transcript and by the size of the library.
SDRF File	E-GEAD-379.sdrf.txt
Comment[AEExperimentType]	ChIP-seq
Comment[BioProject]	PRJDB10255
Comment[Last Update Date]	2021-12-14