Comment[GEAAccession] E-GEAD-395 MAGE-TAB Version 1.1 Investigation Title Transcriptional profiling of ES-2 cells co-cultured with HPMCs or OCAMs Experiment Description Peritoneal dissemination of ovarian cancer (OvCa) arises from the surface of the peritoneum, covered by monolayer of mesothelial cells (MCs). Given that both OvCa cells and MCs are present in the same peritoneal metastatic microenvironment, they may establish cell-to-cell crosstalk or phenotypic alterations including the acquisition of platinum-resistance in OvCa cells. Herein, we report how OvCa-associated mesothelial cells (OCAMs) induce platinum-resistance in OvCa cells through direct cell-to-cell crosstalk. We evaluated mutual associations between OvCa cells and human primary MCs with in vitro coculturing experimental models and in silico omics data analysis. The role of OCAMs was also investigated using clinical samples and in vivo mice models. Results of in vitro experiments show that mesenchymal transition is induced in OCAMs primarily by TGF-b1 stimulation. Experimental Design stimulus or stress design Experimental Factor Name co-culture Experimental Factor Type co-culture Person Last Name Yoshihara Sugiyama Kajiyama Person First Name Masato Mai Hiroaki Person Affiliation Nagoya University Graduate School of Medicine Person Roles submitter submitter submitter Public Release Date 2020-09-18 PubMed ID 31904865 Protocol Name P-GEAD-636 P-GEAD-637 P-GEAD-638 P-GEAD-639 P-GEAD-640 P-GEAD-641 Protocol Type sample collection protocol nucleic acid extraction protocol nucleic acid labeling protocol nucleic acid hybridization to array protocol array scanning and feature extraction protocol normalization data transformation protocol Protocol Description OvCa cells cocultured with untreated HPMCs or TGF-b1-treated HPMCs, we isolated only GFP-labeled ES-2 cells using FACS after cocultures Qiagen RNeasy kit 100ng of total RNA were amplified and labeled with Cy3 using Low Input Quick Amp Labeling Kit, One-Color and RNA Spike-In Kit. Hybridization was performed using Gene Expression Hybridization Kit with 600 ng Cyanine 3-labeled. The arrays were hybridized at 65 degrees C for 17 hours. Microarray slides were scanned in an Agilent SureScan G4900DA Microarray Scanner at 3 micron resolution. The scanned images were analyzed with Feature Extraction Software 11.5.1.1(Agilent) using default Normalization was performed using Agilent GeneSpring GX version 14.8(75 percentile shift) SDRF File E-GEAD-395.sdrf.txt Comment[Number of channel] single-channel Comment[Array Design REF] A-GEOD-20844 Comment[AEExperimentType] transcription profiling by array Comment[BioProject] PRJDB10587 Comment[Last Update Date] 2020-09-18