Comment[GEAAccession]	E-GEAD-400
MAGE-TAB Version	1.1
Investigation Title	Changes in DNA methylation of mouse ovarian granulosa cells during follicular development
Experiment Description	Changes in DNA methylation status of mouse ovarian granulosa cells during follicular development were analyzed by MeDIP-seq. Genomic DNA was obtained from granulosa cells of secondary follicles, granulosa cells in antral follicles (3-week mice not treated with eCG) and granulosa cells in preovulatory follicles (at 48 h after eCG injection) of mouse ovaries.
Experimental Design	development or differentiation design
Experimental Factor Name	strain
Experimental Factor Type	strain
Person Last Name	Kawai
Person First Name	Tomoko
Person Affiliation	Reproductive Biology and Endocrinology, Program of Food and AgriLife Science, Graduate School of Integrated Sciences for Life, Hiroshima University
Person Roles	submitter
Public Release Date	2020-12-10
Protocol Name	P-GEAD-665	P-GEAD-666	P-GEAD-667	P-GEAD-668	P-GEAD-669
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	Granulosa cells was collected from mouse secondary follicles, antral follicles and preovulatory follicles	Genomic DNA was obtained using QIAamp DNA Blood Mini Kit (Qiagen).	One ug of DNA was used as an input sample and treated with MethylMiner Methylated DNA Enrichment kit (Invitrogen).	TruSeq DNA HT Sample Prep kit (Illumina) was used for the Paired End library. Size of the insert for paired reads is 300.	CpG density normalized rms values as specified by the type parameter
SDRF File	E-GEAD-400.sdrf.txt
Comment[AEExperimentType]	MeDIP-seq
Comment[BioProject]	PRJDB10434
Comment[Last Update Date]	2020-12-10