Comment[GEAAccession]	E-GEAD-404
MAGE-TAB Version	1.1
Investigation Title	RNA-seq for identifying the downstream genes of b2-adrenergic receptor signaling in mouse macrophages
Experiment Description	RNA-seq was performed for three different in vitro macrophage models to identify the critical gene that mediates the anti-inflammatory effect of b2 adrenergic receptor signaling. Three different models are as follows: (i) RAW 264.7 cells treated with vehicle, norepinephrine or norepinephrine + butoxamine (b2 antagonist), (ii) RAW 264.7 cells treated with vehicle or salbutamol (b2 agonist), (iii) Peritoneal macrophages treated with vehicle or salbutamol.
Experimental Design	stimulus or stress design
Experimental Factor Name	cell_type	chem_administration
Experimental Factor Type	cell_type	chem_administration
Person Last Name	Hasegawa
Person First Name	Sho
Person Affiliation	Division of CKD Pathophysiology Division of Nephrology and Endocrinology, University of Tokyo
Person Roles	submitter
Public Release Date	2023-05-12
PubMed ID	33875568
Protocol Name	P-GEAD-686	P-GEAD-687	P-GEAD-688	P-GEAD-689	P-GEAD-690
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	RAW 264.7 cells or peritoneal macrophages treated with medication for 4h were collected.	Total RNA was isolated using the RNeasy Mini Kit (74106, Qiagen, Hilden, Germany).	Poly (A)-containing mRNA molecules were isolated from the total RNA, then converted to cDNA with poly A primers using a TruSeq RNA Sample Preparation kit v2 (Illumina).	High-throughput sequencing was performed using a Hiseq2500 (Illumina) system.	Sequenced paired-end reads were mapped onto the mouse genome build mm10 using hisat2 with the parameter '-q --dta-cufflinks'. The SAM file was converted into BAM format and the fragments per kilobase of transcript per million mapped fragments (FPKM) were subsequently calculated using cuffdiff and cummerBund.
SDRF File	E-GEAD-404.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB10719
Comment[Last Update Date]	2023-05-12