Comment[GEAAccession]	E-GEAD-413
MAGE-TAB Version	1.1
Investigation Title	Transcriptome analysis of human corneal epithelial cell with wild type, K4DT, SV40serum, SV40noserum
Experiment Description	These data is the sequence from which the adapter sequence has been removed using software called PEAT. In this study, we compared the biological characteristics of primary and immortalized corneal epithelial cells with the expression of Simian virus 40 large T antigen (SV40) or combinatorial expression of mutant cyclin dependent kinase 4 (CDK4) and Cyclin D1 and telomere reverse transcriptase (TERT). Furthermore, we compared the effect of cell culture condition to transcriptome with SV40 cells with and without serum containing medium. We obtained the whole poly A tailed mRNA expression profiling with RNA-Seq among primary, immortalized cell with combinatorial expression of mutant CDK4, Cyclin D1, and TERT, and immortalized SV40 with serum medium, immortalized SV40 cell with no serum.
Experimental Design	cell component comparison design	genotyping design	all pairs
Experimental Factor Name	genotype
Experimental Factor Type	genotype
Person Last Name	Fukuda
Person First Name	Tomokazu
Person Affiliation	Laboratory of Cell Engineering and Molecular Genetics, Graduate School of Science and Engineering, Iwate University
Person Roles	submitter
Public Release Date	2021-02-24
Protocol Name	P-GEAD-732	P-GEAD-733	P-GEAD-734	P-GEAD-735	P-GEAD-736
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	RNA-seq	Total RNA	polyA	HiseqX	Quality control of short reads was performed using the FastQC tool . Adapter sequences were removed using the PEAT version 1.2 tool.
SDRF File	E-GEAD-413.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB10909
Comment[Last Update Date]	2021-02-24