Comment[GEAAccession]	E-GEAD-435
MAGE-TAB Version	1.1
Investigation Title	Expression profiles of mouse bonemarrow-derived hepetopoietic progenitors and leukemia cells
Experiment Description	Cells were harvested from the bone marrow of C57BL/6J mice. Normal hematopoietic progenitors were prepared by c-kit positive cell selection using magnetic beads. Leukemia cells induced by ectopic epression of MYC and HOXA9 or MYC and MEIS1 combinations were cultured ex vivo for a couple of passages to remove normal hematopietic cells. RNA sapmles of these cells were analyzed by mRNA-seq.
Experimental Design	cell type comparison design
Experimental Factor Name	cell line
Experimental Factor Type	cell line
Person Last Name	Kanai	Yokoyama
Person First Name	Akinori	Akihiko
Person Affiliation	Tsuruoka Metabolomics Laboratory, National Cancer Center	
Person Roles	submitter	submitter
Public Release Date	2021-07-28
Protocol Name	P-GEAD-850	P-GEAD-851	P-GEAD-852	P-GEAD-853	P-GEAD-854
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	Cells were harvested from the bone marrow of 4weeks-old female C57BL/6J mouse. Normal hematopoietic progenitors were prepared by c-kit positive cell selection using magnetic beads. cKit positive cells were first infected with retrovirus for the combinations of MYC and HOXA9 or MYC and MEIS1. and transplanted into syngeneic mice. Leukemia cells were prepared from the bonemarrow of leukemia-bearing mice and were cultured ex vivo for a couple of passages to remove normal hematopietic cells. RNA samples of these cells were analyzed by mRNA-seq.	Total RNA was prepared using the RNeasy kit	The libralies were constructed using a SureSelect Strand Specific RNA Library Prep Kit (Agilent) following the manufacturer's instructions.	The libraries were sequenced for 51 cycles on an Illumina HiSeq 2500 sequencer with 51-bp single-end reads.	Sequenced reads were mapped to human genome assembly mm9 using CASAVA 1.8.2 and gene expression was normalized as reads per kilo base of exon per million mapped (RPKM)
SDRF File	E-GEAD-435.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB11714
Comment[Last Update Date]	2021-07-28