Comment[GEAAccession]	E-GEAD-436
MAGE-TAB Version	1.1
Investigation Title	Expression profiles of various human leukemia cell lines
Experiment Description	K562, HB1119, MV4-11, RS4-11, ML-2, THP1, and EOL1 cells were  analysed by mRNA-seq.
Experimental Design	cell type comparison design
Experimental Factor Name	cell line
Experimental Factor Type	cell line
Person Last Name	Kanai	Yokoyama
Person First Name	Akinori	Akihiko
Person Affiliation	Tsuruoka Metabolomics Laboratory, National Cancer Center	
Person Roles	submitter	submitter
Public Release Date	2021-07-28
Protocol Name	P-GEAD-855	P-GEAD-856	P-GEAD-857	P-GEAD-858	P-GEAD-859
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	K562, HB1119, RS4-11, ML-2, THP1, and EOL1 cells were cultured in RPMI 1640 medium supplemented with 10% FBS and PS. MV4-11 cells were cultured in Iscove's Modified Dulbecco's Media supplemented with 10% FBS and PS. Cell culture was performed in 5% CO2 37 degree C. RNA samples of these cells were analyzed by mRNA-seq.	Total RNA was prepared using the RNeasy kit	The libralies were constructed using a SureSelect Strand Specific RNA Library Prep Kit (Agilent) following the manufacturer's instructions.	The libraries of K562 were sequenced for 36 cycles on an Illumina GAIIx sequencer with 36-bp single-end reads. The libraries of HB1119, MV4-11, RS4-11, ML-2, THP1, and EOL1 were sequenced for 51 cycles on an Illumina HiSeq 2500 sequencer with 51-bp single-end reads.	Sequenced reads were mapped to human genome assembly hg19 using CASAVA 1.8.2 and gene expression was normalized as reads per kilo base of exon per million mapped (RPKM)
SDRF File	E-GEAD-436.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB11729
Comment[Last Update Date]	2021-07-28