Comment[GEAAccession]	E-GEAD-442
MAGE-TAB Version	1.1
Investigation Title	Analysis of RNA editing ratios in Adar1 mutant mice
Experiment Description	Adenosine-to-inosine RNA editing is catalyzed by ADAR1 and ADAR2 in mammals. ADAR1 has two isoforms, short p110 and long p150. Although both ADAR1 KO mice and ADAR1 KI mice that harbor the editing-inactive E861A point mutation are embryonically lethal, this lethality can be rescued by concurrent deletion of MDA5. To elucidate the effect of a mutation associated with Aicardi-Goutieres syndrome, we generated knock-in mouse model by inserting an AGS-associated point mutation in a catalytic deaminase domain of ADAR1. Then, RNA editing pattern in brain and lung was compared with wild-type mice.
Experimental Design	case control design
Experimental Factor Name	genotype
Experimental Factor Type	genotype
Person Last Name	Kato	Nakahama
Person First Name	Yuki	Taisuke
Person Affiliation	Osaka University	
Person Roles	submitter	submitter
Public Release Date	2021-07-07
Protocol Name	P-GEAD-885	P-GEAD-886	P-GEAD-887	P-GEAD-888	P-GEAD-889
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	Nuclei were isolated and purified from flash-frozen mouse brains as previously reported with some modification (Johnson et al., Nat Med 23: 1203-1214, 2017; Hu et al., Mol Cell 68: 1006-1015 e1007, 2017).	5000 single nuclei were  loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol.	Single-cell RNA-seq libraries were obtained following the 10x Genomics recommended protocol, using the reagents included in the Chromium Single Cell 3' Reagent Kit v3.1.	All single-cell RNA-sequencing was performed with an Illumina NovaSeq 6000 with the following setup: paired-end, read1 length 28bp, read2 length 91bp.	Raw count data created with Cell Ranger were uploaded.
SDRF File	E-GEAD-442.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA from single cells
Comment[BioProject]	PRJDB11635
Comment[Last Update Date]	2021-07-07