Comment[GEAAccession]	E-GEAD-444
MAGE-TAB Version	1.1
Investigation Title	TGF-beta1 induces mesothelial-mesenchymal transition in human peritoneal mesothelial cells
Experiment Description	Human primary peritoneal mesothelial cells isolated from tumor-free human omentum were used. Mesothelial cells were cultured on collagen-coated plates in RPMI-1640 media supplemented with 10% FBS and penicillin/streptomycin until confluence and then exposed to TGF-beta1 (10 ng/ml) in low-serum (1% FBS) for 72 hours. Our studies revealed that mesothelial cells, originally functioning as a barrier to the peritoneum, co-organize the tumor microenvironment in the peritoneal dissemination. In peritoneal dissemination, mesothelial cells consist part of the cancer-associated fibroblasts. TGF-beta1 derived from ovarian cancer cells induces mesenchymal changes in mesothelial cells, which enhanced the adhesion of ovarian cancer cells to the peritoneum and their resistance to platinum-based chemotherapy by interacting with each other.
Experimental Design	compound treatment design	translational bias design	replicate design
Experimental Factor Name	treatment
Experimental Factor Type	treatment
Person Last Name	Mogi	Kitami	Yoshihara	Kajiyama
Person First Name	Kazumasa	Kazuhisa	Masato	Hiroaki
Person Affiliation	Department of obstetrics and gynecology, Nagoya University, Graduate School of Medicine			
Person Roles	submitter	submitter	submitter	submitter
Public Release Date	2025-06-27
Protocol Name	P-GEAD-895	P-GEAD-896	P-GEAD-897	P-GEAD-898	P-GEAD-899
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	After TGF-beta1 was added to primary peritoneal mesothelial cells cultured confluently in collagen-coated dish for 72 hours, samples were collected in QIAGEN RLT buffer.	Total RNA was extracted from human omental mesothelial cells using QIAGEN RNeasy Mini kit.	A total of 2 micro gram of RNA was used for construction.	The libraries were sequenced for 300 cycles.	Remove reads containing low quality (Qscore<= 5) base which is over 50% of the total base.
SDRF File	E-GEAD-444.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB11880
Comment[Last Update Date]	2025-06-27