Comment[GEAAccession]	E-GEAD-448
MAGE-TAB Version	1.1
Investigation Title	The effect of conditioned medium from senescent cells on gene expression
Experiment Description	We analyzed the effect of conditioned medium from senescent cells on gene expression in the Madin-Darby canine kidney (MDCK) cell line.
Experimental Design	stimulus or stress design
Experimental Factor Name	Condition
Experimental Factor Type	Condition
Person Last Name	Miyata	Takahashi
Person First Name	Kenichi	Akiko
Person Affiliation	Project for Cellular Senescence, Cancer Institute, Japanese Foundation for Cancer Research	
Person Roles	submitter	submitter
Public Release Date	2022-06-21
Protocol Name	P-GEAD-916	P-GEAD-917	P-GEAD-918	P-GEAD-919	P-GEAD-920	P-GEAD-921
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid labeling protocol	nucleic acid hybridization to array protocol	array scanning and feature extraction protocol	normalization data transformation protocol
Protocol Description	MDCK cells were cultured in Dulbeccos Modified Eagles (DME) medium supplemented with 10% FBS and penicillin/streptomycin at 5% CO2 condition.	Total RNA was extracted from MDCK-pTR GFP-RasV12 cells by mirVana miRNA Isolation Kit according to the manufacturers instructions.	labeled cRNA were prepared from 1~5 ug total RNA using the Agilents Quick Amp Labeling Kit performed by Macrogen Japan Corporation.	Following fragmentation, 1.65 ug of cRNA were hybridized to the Agilent expression microarray according to the protocols provided by the manufacturer performed by Macrogen Japan Corporation.	Arrays were scanned using the Agilent Technologies G4900DA SG12494263 performed by Macrogen Japan Corporation	Array data export processing and analysis was performed using Agilent Feature Extraction v11.0.1.1 performed by Macrogen Japan Corporation.
SDRF File	E-GEAD-448.sdrf.txt
Comment[Number of channel]	single-channel
Comment[Array Design REF]	A-GEOD-15379
Comment[AEExperimentType]	transcription profiling by array
Comment[BioProject]	PRJDB12109
Comment[Last Update Date]	2022-06-21