Comment[GEAAccession]	E-GEAD-453
MAGE-TAB Version	1.1
Investigation Title	Transcriptome of pho80
Experiment Description	To identify the phosphate starvation responsive genes, we analyzed the transcriptome of the PHO80-deletion and wild-type strains cultivated in a high-phosphate synthetic medium in Saccharomyces cerevisiae.
Experimental Design	genetic modification design
Experimental Factor Name	genetic_modification
Experimental Factor Type	genetic_modification
Person Last Name	Mukai
Person First Name	Yukio
Person Affiliation	Laboratory of Environmental Microbiology Department of Frontier Bioscience Faculty of Biosciences, Nagahama Institute of Bio-Science and Technology
Person Roles	submitter
Public Release Date	2023-08-22
PubMed ID	37574219
Protocol Name	P-GEAD-943	P-GEAD-944	P-GEAD-945	P-GEAD-946	P-GEAD-947
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	Yeast cells of the PHO80-deletion and wild-type strains were cultivated at 30 degrees in a high-phosphate synthetic medium.	Total RNAs were extracted from yeast cells using RNeasy Mini Kit (Qiagen) and ribosomal RNAs were removed using riboPOOL (siTOOLs Biotech).	A single-stranded circularized DNA library was generated using MGIEasy RNA Directional Library Prep Set and MGIEasy circularization Kit (MGI Tech Co., Ltd.).	RNA-seq analysis was performed using DNBSEQ-G400 (2 x 100 bp paired-end reads) by Bioengineering Lab. Co., Ltd..	The reads were mapped to the reference sequence (S288C genome) using hisat2. The gene expression abundance was normalized by TPM (Transcripts Per Million).
SDRF File	E-GEAD-453.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB12313
Comment[Last Update Date]	2023-08-22