Comment[GEAAccession]	E-GEAD-455
MAGE-TAB Version	1.1
Investigation Title	Transcriptional profile of grape cultured cells exposed to electrical stimulation
Experiment Description	Microarray analysis of grape cultured cells exposed to electrical stimulation was performed. Briefly, electrical stimulation was applied by inserting two electrodes (0.5 mm diameter) into the cell mass and connecting them to a solar panel under a fluorescent lamp. Untreated grape cultured cells were used as control. After the electrical stimulation for 4 h, the cell mass was subjected to microarray analysis using GeneChip Vitis vinifera (Grape) Genome Array (Affymetrix).
Experimental Design	stimulus or stress design
Experimental Factor Name	treatment
Experimental Factor Type	treatment
Person Last Name	Suzuki
Person First Name	Shunji
Person Affiliation	University of Yamanashi
Person Roles	submitter
Public Release Date	2021-10-07
Protocol Name	P-GEAD-953	P-GEAD-954	P-GEAD-955	P-GEAD-956	P-GEAD-957	P-GEAD-958	P-GEAD-959	P-GEAD-960	P-GEAD-961
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid labeling protocol	nucleic acid hybridization to array protocol	array scanning and feature extraction protocol	normalization data transformation protocol	growth protocol	treatment protocol	conversion protocol
Protocol Description	Grape cultured cells prepared from meristems of V. vinifera cv. Koshu were subjected to electrical stimulation.	Total RNA isolation from the cell mass was performed with a Fruit-mate for RNA Purification (Takara, Otsu, Japan), followed by a NucleoSpin RNA Plant (Takara).	Biotin-labeled cRNA synthesis using GeneChip 3IVT PLUS Reagent Kit (Affymetrix) was performed according to the manufacturer instructions.	Hybridization using GeneChip Hybridization, Wash and Stain Kit (Affymetrix) was performed according to the manufacturer instructions.	Signal detection using GeneChip Scanner 3000 7G (Affymetrix) was performed according to the manufacturer instructions.	Analysis of the signal intensity of each spot and signal evaluation and normalization were performed using Affymetrix GeneChip Command Console Software 4.0 (Affymetrix) and Affymetrix Expression Console Software 1.4.	Grapevine cultured cells exposed to electrical stimulation were grown at 27 ºC for 4 h on modified Gamborg B5 agar plates.	Electrical stimulation was applied by inserting two electrodes (0.5 mm diameter) into the cell mass and connecting them to a solar panel under a fluorescent lamp.	Biotin-labeled cRNA synthesis using GeneChip 3IVT PLUS Reagent Kit (Affymetrix) was performed according to the manufacturer instructions.
SDRF File	E-GEAD-455.sdrf.txt
Comment[Number of channel]	single-channel
Comment[Array Design REF]	A-AFFY-78
Comment[AEExperimentType]	transcription profiling by array
Comment[BioProject]	PRJDB12347
Comment[Last Update Date]	2021-10-07