Comment[GEAAccession]	E-GEAD-456
MAGE-TAB Version	1.1
Investigation Title	Change in gene expression in the compound-treated T cells
Experiment Description	To reveal the mechanisms underlying the effect of our compound on T cells, we compared the gene expression between non-treated T cells and our compound-treated T cells.
Experimental Design	compound treatment design
Experimental Factor Name	compound
Experimental Factor Type	compound
Person Last Name	Muraoka
Person First Name	Daisuke
Person Affiliation	Oncology, Nagasaki University
Person Roles	submitter
Public Release Date	2025-09-01
Protocol Name	P-GEAD-962	P-GEAD-963	P-GEAD-964	P-GEAD-965	P-GEAD-966
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	Splenocytes isolated from TCR transgenic mice were treated with specific-antigen and compound for 24 hours	Total RNA was isolated using the Rneasy Kit (Qiagen) according to the manufacturer's instructions	Collibri 3mRNA Library Prep kit	Miseq (Illumina)	mouse mm10 genome using TopHat2
SDRF File	E-GEAD-456.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB12238
Comment[Last Update Date]	2025-09-01