Comment[GEAAccession]	E-GEAD-457
MAGE-TAB Version	1.1
Investigation Title	Glioma gene expression with methionine
Experiment Description	Malignant transformation of recurrence of glioblastoma multiform is accompanied by methionine accumulation. This project aims to understand how methionine metabolism is associated with glioma-initiating cells.
Experimental Design	stimulus or stress design
Experimental Factor Name	treatment
Experimental Factor Type	treatment
Person Last Name	Kikuchi	Yokogami
Person First Name	Taisei	Kiyotaka
Person Affiliation	Faculty of Medicine, University of Miyazaki	
Person Roles	submitter	submitter
Public Release Date	2021-10-27
Protocol Name	P-GEAD-967	P-GEAD-968	P-GEAD-969	P-GEAD-970	P-GEAD-971	P-GEAD-972
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid labeling protocol	nucleic acid hybridization to array protocol	array scanning and feature extraction protocol	normalization data transformation protocol
Protocol Description	Cultured cells are collected using the standard protocol.	RNA from cultured tumor cells was extracted with RNeasy Plus Mini Kit (Qiagen).	Biotin labelling of fragmented cDNA was performed using biotin labelling system (GeneChip WT PLUS Reagent Kit, Thermo Fisher Scientific).	Labelled probes were hybridized to the Human Genome Clariom D Array with the GeneChip Scanner 3000 7G (Affymetrix).	Expression data of mRNA were extracted image files using GeneSpring GX 14.9.1 (Agilent Technologies).	Normalization and expression value calculations were performed using GeneSpring system.
SDRF File	E-GEAD-457.sdrf.txt
Comment[Number of channel]	single-channel
Comment[Array Design REF]	A-GEOD-17586
Comment[AEExperimentType]	transcription profiling by array
Comment[BioProject]	PRJDB12471
Comment[Last Update Date]	2021-10-27