Comment[GEAAccession]	E-GEAD-467
MAGE-TAB Version	1.1
Investigation Title	Gene expression change after treatment of H3K27me3 inhibitors or FBI siRNA.
Experiment Description	Neural stem cell from E10 mice were treated with Gsk343, GskJ4 or both inhibitors for three days. Neural stem cell from E10 mice were treated with control or FBI siRNAs for three days. RNA from these samples were collected and RNA-seq was performed.
Experimental Design	case control design	binding site identification design	replicate design
Experimental Factor Name	treatment
Experimental Factor Type	treatment
Person Last Name	wu
Person First Name	quan
Person Affiliation	RIKEN BDR
Person Roles	submitter
Public Release Date	2021-12-21
Protocol Name	P-GEAD-1019	P-GEAD-1020	P-GEAD-1021	P-GEAD-1022	P-GEAD-1023
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	Qiagen RNeasy Mini Kits	Qiagen RNeasy Mini Kits	TruSeq Stranded mRNA library Pre Kit	HiSeq 1500	Stringtie-TCC
SDRF File	E-GEAD-467.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB9278
Comment[Last Update Date]	2021-12-21