Comment[GEAAccession]	E-GEAD-468
MAGE-TAB Version	1.1
Investigation Title	Single cell RNA seq of tamoxifen treated MCF-7 cells at 0, 3, 6, and 9 weeks
Experiment Description	The process of acquiring drug resistance in cancer cells can be understood as a process of rewiring of molecular network stimulated by the change of extracellular environment. To elucidate the cell-to-cell variability in breast cancer cells during the acquiring process of resistance to estrogen receptor antagonists, Human breast cancer MCF-7 cells were exposed to 1 uM of tamoxifen for 3, 6, and 9 weeks, and then we conducted single-cell RNA sequencing.
Experimental Design	compound treatment design	time series design
Experimental Factor Name	time
Experimental Factor Type	time
Person Last Name	Magi
Person First Name	Shigeyuki
Person Affiliation	Laboratory of Cell Systems, Institute for Protein Research, Osaka University
Person Roles	submitter
Public Release Date	2021-12-24
Publication DOI	https://doi.org/10.1038/s41598-021-97887-z
Protocol Name	P-GEAD-1024	P-GEAD-1025	P-GEAD-1026	P-GEAD-1027	P-GEAD-1028
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	MCF-7 cells treated with or without continuous tamoxifen were trypsinized and collected following dilution with the culture medium. The cells were then washed twice with cold PBS and stained with Hechest33342 (5 microgram/mL) and PI (1 microgram/mL) for 15 min	The cells were diluted to a concentration of 20,000 cells/mL and loaded into the ICELL-8 single-cell system.	cDNA was prepared using 3DE reagents (Takara Bio) according to the manufacturer's instructions.	100-bp paired-end sequencing on the Illumina HiSeq 3000 platform (Illumina, CA, U.S.A.)	Mapping of sequence reads to the human reference genome (version GRCh38) sequence and counting genes were carried out using the mappa and hanta software (Takara Bio).
SDRF File	E-GEAD-468.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA from single cells
Comment[BioProject]	PRJDB8870
Comment[Last Update Date]	2021-12-24