Comment[GEAAccession]	E-GEAD-492
MAGE-TAB Version	1.1
Investigation Title	Effects of E7386 in CRC organiods and CAFs
Experiment Description	Different types of reactions, such as alterations in the glucose and amino acid metabolic pathways, stimulation of stress responses and NK-cell mediated cytotoxicity, and components in the Wnt/beta-catenin signaling pathway, to E7386 in the CRC organoids and corresponding cancer-associated fibroblasts (CAFs) were observed under conditions with decreased cell viability.
Experimental Design	cell type comparison design	dose response design	genotype design	time series design	validation by real time PCR design
Experimental Factor Name	dose	time	cell_type	culture_type
Experimental Factor Type	dose	time	cell_type	culture_type
Person Last Name	Imai
Person First Name	Toshio
Person Affiliation	Department of Cancer Model Development, National Cancer Center Research Institute
Person Roles	submitter
Public Release Date	2022-04-19
Protocol Name	P-GEAD-1146	P-GEAD-1147	P-GEAD-1148	P-GEAD-1149	P-GEAD-1150	P-GEAD-1151
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid labeling protocol	nucleic acid hybridization to array protocol	array scanning and feature extraction protocol	normalization data transformation protocol
Protocol Description	Organoids/CAFs were harvested by direct lysis in the vessel.	RNeasy Micro Kit	Cy3	As per Agilent instructions	As per Agilent instructions	As per Agilent instructions
SDRF File	E-GEAD-492.sdrf.txt
Comment[Number of channel]	single-channel
Comment[Array Design REF]	A-GEOD-21185
Comment[AEExperimentType]	transcription profiling by array
Comment[BioProject]	PRJDB13321
Comment[Last Update Date]	2022-04-19