Comment[GEAAccession]	E-GEAD-493
MAGE-TAB Version	1.1
Investigation Title	Comprehensive gene expression analysis by DNA microarray
Experiment Description	Comprehensive gene expression data of various samples are acquired by our proprietary DNA microarray, and we mainly perform comparative analysis and extraction of marker genes.
Experimental Design	compound treatment design
Experimental Factor Name	missing
Experimental Factor Type	missing
Person Last Name	Morisawa
Person First Name	Gaku
Person Affiliation	Fukushima Medical University
Person Roles	submitter
Public Release Date	2023-11-18
Protocol Name	P-GEAD-1152	P-GEAD-1153	P-GEAD-1154	P-GEAD-1155	P-GEAD-1156	P-GEAD-1157
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid labeling protocol	nucleic acid hybridization to array protocol	array scanning and feature extraction protocol	normalization data transformation protocol
Protocol Description	Besides commercially available specimens, solid tumor tissues obtained from surgical or biopsy specimens, and ascites and pleural fluids, were acquired from patients with cancer at Fukushima Medical University Hospital, upon providing informed consent. Cell lines were purchased from a cell bank.	Total RNA was extracted from the cells using ISOGEN (Nippon Gene Co., Ltd., Tokyo, Japan)	Total RNA (5 ????????g) was labeled using SuperScript II (Invitrogen; Thermo Fisher Scientific, Inc.) and cyanine 5-dUTP (PerkinElmer, Inc., Waltham, MA, USA) for samples, or cyanine 3-dUTP (PerkinElmer, Inc.) for human common reference RNA, which was prepared by mixing equal amounts of total RNA extracted from 22 cell lines.	Hybridization was performed with a labeling and hybridization kit (MicroDiagnostic, Inc.).	Signals were measured with a GenePix 4000B scanner (Molecular Devices, LLC, Sunnyvale, CA, USA) and were converted into primary expression ratios (ratio of cyanine-5 intensity of each sample to cyanine-3 intensity of the human common reference RNA).????????	Each ratio was normalized through multiplication using normalization factors and Gene Pix Pro 3.0 software (Molecular Devices, LLC).
SDRF File	E-GEAD-493.sdrf.txt
Comment[Number of channel]	dual-channel
Comment[Array Design REF]	A-GEAD-15
Comment[AEExperimentType]	transcription profiling by array
Comment[BioProject]	PRJDB13362
Comment[Last Update Date]	2023-11-18