Comment[GEAAccession]	E-GEAD-496
MAGE-TAB Version	1.1
Investigation Title	RNA-seq profiling of human renal interstitial progenitor cells and posterior primitive streak cells from iPSCs
Experiment Description	To characterize the gene expression profile of human renal interstitial progenitor cells and posterior primitive streak cells from iPSCs, we performed RNA-seq analysis.
Experimental Design	cell type comparison design
Experimental Factor Name	cell_type
Experimental Factor Type	cell_type
Person Last Name	Tsujimoto
Person First Name	Hiraku
Person Affiliation	Osafune Laboratory, Kyoto University, Center for iPS Cell Research and Application (CiRA)
Person Roles	submitter
Public Release Date	2024-01-23
PubMed ID	38237600
Protocol Name	P-GEAD-1168	P-GEAD-1169	P-GEAD-1170	P-GEAD-1171	P-GEAD-1172
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	Human renal interstitial progenitor cells and posterior primitive streak cells were induced from iPSCs	Total RNA was isolated using the RNeasy Mini Kit.	After poly(A)(+) RNA enrichment using a NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs), double-stranded cDNA libraries (RNA-seq libraries) were prepared using a SMARTer Stranded RNA-Seq Kit (Clontech) according to the manufacturer instructions.	RNA-seq libraries were sequenced using paired end reads (50 nt of read 1 and 25 nt of read 2) on a NextSeq 500 instrument (Illumina).	Transcript quantification was performed using Salmon (Patro et al., 2017) with GRCh38.p13 cDNA gene annotation (GENCODE).
SDRF File	E-GEAD-496.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB13281
Comment[Last Update Date]	2024-01-23