Comment[GEAAccession]	E-GEAD-506
MAGE-TAB Version	1.1
Investigation Title	Comparison of therapeutic effects of mesenchymal stem cells from superficial and deep subcutaneous adipose tissues
Experiment Description	We extracted mesenchymal stem cells (MSCs) derived from superficial and deep subcutaneous adipose tissues (Sup-MSCs and Deep-MSCs, respectively), and compared their functional difference using RNA sequencing.
Experimental Design	cell type comparison design
Experimental Factor Name	tissue
Experimental Factor Type	tissue
Person Last Name	ishiuchi
Person First Name	naoki
Person Affiliation	Department of Nephrology, Hiroshima University Hospital
Person Roles	submitter
Public Release Date	2023-02-13
Protocol Name	P-GEAD-1221	P-GEAD-1222	P-GEAD-1223	P-GEAD-1224	P-GEAD-1225
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	MSCs were isolated from superficial and deep subcutaneous abdominal adipose tissues collected from patients who underwent breast reconstruction, herein referred to as \"Sup-MSCs\" and \"Deep-MSCs,\" respectively.These cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich, St. Louis, MO) containing 10% FBS (Sigma-Aldrich) and were used in all experiments up to five passages. Each patient had provided written informed consent.	We processed according to TruSeq Stranded Total RNA Sample Prep Guide, Part #15031048 Rev. E	A library was independently prepared with 1 ?g total RNA for each sample using the Illumina TruSeq Stranded mRNA Sample Prep Kit (Illumina, Inc., San Diego, CA).	The qualified libraries were sequenced using an Illumina NovaSeq (Illumina, Inc.) with paired-end (2?100 bp) reads.	The expression abundances of transcripts and genes were calculated as the read count or fragments per kilobase of exon per million mapped read value per sample.
SDRF File	E-GEAD-506.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB13831
Comment[Last Update Date]	2023-02-13