Comment[GEAAccession]	E-GEAD-529
MAGE-TAB Version	1.1
Investigation Title	scRNA-seq of spleen about cholinergic anti-inflammatory pathway
Experiment Description	Purpose: The cholinergic anti-inflammatory pathway is an immune response mediated by the nervous system. CD4 T cells, macrophages, and alpha 7nAChR are known to be essential in this pathway, but this signal transduction has not been demonstrated in vivo. The aim of this study is to identify the cells and genes that receive cholinergic signals via alpha 7nAChR on the spleen cells. Methods: Spleens removed from wild-type (10 weeks-old, C57BL6J, male) mice treated with GTS-21 (20 mg/kg) with or without LPS (5 mg/kg) 4 hours. Then scRNA-seq was performed. Results: GTS-21 or LPS treatment did not alter the proportion of the cells or the gene expression in macrophages. Ligand-receptor analysis revealed increased ligand-receptor pairs between macrophages among spleen cells. Conclusion: Our findings suggested that cholinergic signals via alpha 7nAChR is increases macrophage-macrophage interactions in the spleen and exerts anti-inflammatory effects.
Experimental Design	injury design
Experimental Factor Name	compound
Experimental Factor Type	compound
Person Last Name	Nakamura	Inoue	Matsumoto
Person First Name	Yasuna	Tsuyoshi	Hirotaka
Person Affiliation	Nagasaki University		
Person Roles	submitter	submitter	submitter
Public Release Date	2024-07-29
Protocol Name	P-GEAD-1363	P-GEAD-1364	P-GEAD-1365	P-GEAD-1366	P-GEAD-1367
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	Wild-type (C57BL6J, 10 weeks old, male) mice treated with GTS-21with or without LPS four hours, then euthanized and harvested spleens.	Spleens are single celled with digestion buffer, then 7000 cells were loaded onto 10x Genomics Chromium instrument to create single-cell Gel Bead-in-Emulsions.	cDNA libraries were constructed using 10x Chromium Single Cell 3' Reagent Kits v3.1.	Libraries were seform.quenced using Illumina NovaSeq platform.	We used Seurat v.4.0.6 \"NormalizeData\" function.
SDRF File	E-GEAD-529.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA from single cells
Comment[BioProject]	PRJDB14097
Comment[Last Update Date]	2024-07-29