Comment[GEAAccession]	E-GEAD-550
MAGE-TAB Version	1.1
Investigation Title	RNA-sequencing on murine gallbladder tumor organoids (Elf3 wild type vs. Elf3 deletion)
Experiment Description	To elucidate the functional role of Elf3 in gallbladder cancer development, we generated Elf3-deleted gallbladder tumor mouse model (KPCE, Pdx1-Cre; KrasG12D; Trp53R172H; Elf3 f/f). Organoids were generated from the gallbladder epithelium in Elf3-deleted mouse model and control mice (KPC, Pdx1-Cre; KrasG12D; Trp53R172H) and RNA-sequencing was performed on these organoids to clarify underlying mechanism of gallbladder tumor development.
Experimental Design	genotype design
Experimental Factor Name	genotype
Experimental Factor Type	genotype
Person Last Name	Nakamura
Person First Name	Takeharu
Person Affiliation	Department of Gastroenterology and Hepatology, Graduate School of Medicine, Kyoto University
Person Roles	submitter
Public Release Date	2026-09-27
Protocol Name	P-GEAD-1486	P-GEAD-1487	P-GEAD-1488	P-GEAD-1489	P-GEAD-1490
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	We used organoids generated from the gallbladder epithelium in experimental and control mice.	RNA was extracted using RNeasy Mini Kit (QIAGEN).	Library was constructed using TruSeq Stranded mRNA Library Prep Kit (Illumina).	NovaSeq 6000 System User Guide Document # 1000000019358 v02	trimmed mean of M values algorism in edgeR library (reference: mm10)
SDRF File	E-GEAD-550.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB14418
Comment[Last Update Date]	2026-09-27