Comment[GEAAccession]	E-GEAD-551
MAGE-TAB Version	1.1
Investigation Title	single cell RNA-seq of COVID-19 patients (with ARDS, n=8; without ARDS, n=8) and healthy controls (n=5)
Experiment Description	In this study, we aimed to reveal the differentially expressed genes in monocytes derived from peripheral blood mononuclear cells between patients and healthy donors, or between COVID-19 patients with ARDS and without ARDS.
Experimental Design	case control design
Experimental Factor Name	condition
Experimental Factor Type	condition
Person Last Name	Yamaguchi	Kato
Person First Name	Yuta	Yasuhiro
Person Affiliation	Department of Respiratory Medicine and Clinical Immunology, Osaka University Graduate School of Medicine	
Person Roles	submitter	submitter
Public Release Date	2023-03-20
Protocol Name	P-GEAD-1491	P-GEAD-1492	P-GEAD-1493	P-GEAD-1494	P-GEAD-1495	P-GEAD-1496
Protocol Type	1010	1020	1030	1040	1050	1080
Protocol Description	Peripheral blood mononuclear cells for scRNA-seq were obtained from patients with COVID-19 (ARDS complicated, n = 8; non-ARDS complicated, n = 8) and healthy donors (n = 5)	Single-cell suspensions were loaded on a 10x Genomics Chromium Controller.	cDNA with a cell barcode and unique molecular index was produced from each encapsulated cell in an oil droplet by reverse transcription.	The libraries were sequenced on an Illumina NovaSeq 6000 in paired-end mode (read1: 26 bp; read2: 91 bp).	The Seurat package in R (v3.2.2) was used to perform scaling, transformation, clustering, dimensionality reduction, differential expression analysis, and visualization.	missing
SDRF File	E-GEAD-551.sdrf.txt
Comment[Number of channel]	single-channel
Comment[AEExperimentType]	transcription profiling by array
Comment[BioProject]	PRJDB14382
Comment[Last Update Date]	2023-03-20