Comment[GEAAccession]	E-GEAD-552
MAGE-TAB Version	1.1
Investigation Title	RNA-seq and ATAC-seq of healthy donors treated with BNT162b2 mRNA vaccine
Experiment Description	In this study, we aimed to understand the epigenetic changes in monocytes derived from peripheral blood mononuclear cells induced by consecutive mRNA vaccination.
Experimental Design	case control design
Experimental Factor Name	condition
Experimental Factor Type	condition
Person Last Name	Yamaguchi	Kato
Person First Name	Yuta	Yasuhiro
Person Affiliation	Department of Respiratory Medicine and Clinical Immunology, Osaka University Graduate School of Medicine	
Person Roles	submitter	submitter
Public Release Date	2023-03-20
Protocol Name	P-GEAD-1497	P-GEAD-1498	P-GEAD-1499	P-GEAD-1500	P-GEAD-1501	P-GEAD-1502
Protocol Type	1010	1020	1030	1040	1050	1080
Protocol Description	Peripheral blood mononuclear cells for RNA-seq and ATAC-seq were obtained from 5 healthy males before and 1, 20, 22, 49 days after the BNT162b2 mRNA vaccine.	Total RNA and DNA were extracted from monocytes derived from peripheral blood mononuclear cells of healthy males.	Full-length cDNA for RNA-seq was generated using a SMART-Seq HT Kit (Takara Bio, 634436), and libraries for ATAC-seq were prepared using an ATAC-Seq Kit (Active Motif, 53150).	The libraries were sequenced on an Illumina NovaSeq 6000 (RNA-seq, 101-base paired-end mode; ATAC-seq, in 150-base paired-end mode.)	The raw counts of RNA-seq were normalized to transcripts per million (TPM), and the raw read counts of ATAC-seq were normalized to the trimmed mean of M values (TMM).	missing
SDRF File	E-GEAD-552.sdrf.txt
Comment[Number of channel]	single-channel
Comment[Array Design REF]	A-GEAD-21
Comment[AEExperimentType]	transcription profiling by array
Comment[BioProject]	PRJDB14366
Comment[Last Update Date]	2023-03-20