Comment[GEAAccession]	E-GEAD-566
MAGE-TAB Version	1.1
Investigation Title	Transcriptome in the brains of nurse bees and foragers before and after foraging in Apis mellifera
Experiment Description	"To investigate whether the expression levels of AmEcR target genes changes according to foraging behavior or age-polyethism, we performed RNA-seq analysis of the brains of nurse bees and foragers before and after foraging."
Experimental Design	innate behavior design	replicate design
Experimental Factor Name	worker behavior
Experimental Factor Type	worker behavior
Person Last Name	Kohno
Person First Name	Hiroki
Person Affiliation	Laboratory of Physiological Chemistry Department of Biological Sciences Graduate School of Science, The University of Tokyo
Person Roles	submitter
Public Release Date	2023-11-07
PubMed ID	37380789
Protocol Name	P-GEAD-1572	P-GEAD-1573	P-GEAD-1574	P-GEAD-1575	P-GEAD-1576
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	"3 brains per lot (4 lots for each bee sample) were dissected from nurse bees, foragers before / after foraging."	total RNA was extracted from brains homogenated in TRIzol reagent using a Direct-zol RNA kit	Stranded RNA-seq libraries for DNBSEQ were prepared with polyA selection	The libraries were sequenced with MGI tech DNBSEQ G-400	"The adaptor sequences were trimmed using Trimmomatic,and mapped onto the honey bee genome with HISAT2. Transcripts count matrices of genes were obtained using StringTie and prepDE.py."
SDRF File	E-GEAD-566.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB14260
Comment[Last Update Date]	2023-11-07