Comment[GEAAccession]	E-GEAD-603
MAGE-TAB Version	1.1
Investigation Title	scRNA-seq on hPSC-derived bones
Experiment Description	This data was derived from induced bone tissues at 7 and 19 weeks by implantation of hiPSC-derived sclerotome cells beneath the renal capsules of immunodeficient mice. After the cell dissociation for 3 hours, nuclei were extracted from the dissociated cells. For library preparation, Chromium Single cell 3' Library and Gel Bead Kit V3 (10X Genomics) was used. After sequencing with Illumina Novaseq 6000, fastq files were processed by cellranger (v7.0.1). Three necessary data for Seurat pipeline were archived as one tar file.
Experimental Design	development or differentiation design
Experimental Factor Name	dev_stage
Experimental Factor Type	dev_stage
Person Last Name	Tani
Person First Name	Shoichiro
Person Affiliation	Center for Disease Biology and Integrative Medicine Graduate School of Medicine, The University of Tokyo
Person Roles	submitter
Public Release Date	2023-04-14
Publication DOI	10.1016/j.celrep.2023.112276
Protocol Name	P-GEAD-1762	P-GEAD-1763	P-GEAD-1764	P-GEAD-1765	P-GEAD-1766
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	hPSC-derived bones were induced for 7 and 19 weeks. Bone tissues were cut into small pieces and incubated with LiberaseTM or EDTA in PBS to dissociate cells from the tissues.	After making single-cell droplets, reverse transcription was performed.	Chromium Single cell 3' Library and Gel Bead Kit V3	Illumina Novaseq 6000	Fastq files were processed by cellranger (vv7.0.1). For scRNA-seq data with Seurat pipeline, 3 files of filtered_feature_bc_matrix were archived as a tar file.
SDRF File	E-GEAD-603.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA from single cells
Comment[BioProject]	PRJDB13702
Comment[Last Update Date]	2023-04-14