Comment[GEAAccession]	E-GEAD-612
MAGE-TAB Version	1.1
Investigation Title	Spatial transcriptome analysis of the fetal marmoset brain
Experiment Description	Primates have a more developed cerebrum than rodents and have acquired higher-order brain functions. Especially in the fetal brain, the subplate layer, which plays an essential role in brain construction, is transiently thickened. Therefore, we performed Visium spatial transcriptome analysis using marmoset fetal brains to compare the primate brain formation process with mice. The aim is to elucidate primate-specific mechanisms of brain formation through interspecies comparative analysis.
Experimental Design	development or differentiation design
Experimental Factor Name	sampling_site
Experimental Factor Type	sampling_site
Person Last Name	Ohtaka-Maruyama	Moriya-Ito	Sequence
Person First Name	Chiaki	Keiko	Kashiwa
Person Affiliation	Developmental Neuroscience Project, Maruyama Lab		
Person Roles	submitter	submitter	submitter
Public Release Date	2025-06-01
Protocol Name	P-GEAD-1807	P-GEAD-1808	P-GEAD-1809	P-GEAD-1810	P-GEAD-1811
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	The fetus is surgically removed from a pregnant marmoset (98 days old), the brain is isolated and quickly frozen. Fresh-frozen sections were prepared.	Visium Spatial Gene Expression Reagent Kits(10x Genomics)	The sequence library was constructed using the Visium Spatial Gene Expression Reagent Kits(10x Genomics).	Sequencing was performed on the Illumina NovaSeq6000 in paired end mode; Read1:Spatial Barcode, UMI(28 bp), i7 Index:Sample Index(10 bp), i5 Index:Sample Index(10 bp), Read2:Insert(90 bp).	Sequenced reads were mapped to genome assembly by Callithrix jacchus: GCF_009663435.1, using spaceranger v1.3.1(10x Genomics). Slide information; A1_common_marmoset_E105_frontal_parietal_cortex:slide=V11N29-088,area=A1, B1_common_marmoset_E105_temporal_area:slide=V11N29-088,area=B1,  C1_common_marmoset_E105_occipital_cortex:slide=V11N29-088,area=C1, D1_common_marmoset_E105_hippocampus:slide=V11N29-088,area=D1
SDRF File	E-GEAD-612.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB15896
Comment[Last Update Date]	2025-06-01