Comment[GEAAccession]	E-GEAD-625
MAGE-TAB Version	1.1
Investigation Title	Spatial transcriptome analysis by FFPE Visium for mouse nasal cavity.
Experiment Description	We used the Visium spatial transcriptome (ST) platform produced by 10x Genomics to perform ST analysis of the mouse nasal cavity 0 h, 3 h, 6 h, 24 h after intranasal vaccination. The files contain GEX matrix data, json file, tiff file for analysis by SpaceRanger.
Experimental Design	observational design	organism part comparison design	time series design
Experimental Factor Name	treatment
Experimental Factor Type	treatment
Person Last Name	Iwabuchi
Person First Name	Sadahiro
Person Affiliation	Institute of Advanced Medicine, Department of Molecular Pathophysiology, Wakayama Medical University
Person Roles	submitter
Public Release Date	2023-06-27
Protocol Name	P-GEAD-1875	P-GEAD-1876	P-GEAD-1877	P-GEAD-1878	P-GEAD-1879
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	After FFPE blocked in each tissue, we followed the VisiumSpatialGeneExpression_UserGuide.	Visium Spatial Gene Expression Reagent Kits(10x Genomics)	The sequence library was constructed using the Visium Spatial Gene Expression Reagent Kits(10x Genomics).	Sequencing was performed on the Illumina NextSeq550 in paired end mode; Read1:Spatial Barcode, UMI(28 bp), i7 Index:Sample Index(10 bp), i5 Index:Sample Index(10 bp), Read2:Insert(100 bp).	Sequence data was processed by Space Ranger v1.2.1(10x Genomics). Slide information; 0h:slide=V11Y10-342-A1,area=A1, 3h:slide=V11Y10-342-B1,area=B1, 6h:slide=V11Y10-342-C1,area=C1, 24h:slide=V11Y10-342-D1,area=D1
SDRF File	E-GEAD-625.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB16127
Comment[Last Update Date]	2023-06-27