Comment[GEAAccession]	E-GEAD-633
MAGE-TAB Version	1.1
Investigation Title	In situ gene expression data of FF and FFPE mouse brain
Experiment Description	Using  Xenium Analyzer(10x Genomics), In situ gene expression data of FF and FFPE mouse brain were obtained.
Experimental Design	organism part comparison design
Experimental Factor Name	sample_type	sectioning
Experimental Factor Type	sample_type	sectioning
Person Last Name	Suzuki	Sequence
Person First Name	Yutaka	Kashiwa
Person Affiliation	Suzuki Lab  Department of Computational Biology and Medical Sciences Graduate School of Frontier Sciences, The University of Tokyo	
Person Roles	submitter	submitter
Public Release Date	2023-10-10
Protocol Name	P-GEAD-1919	P-GEAD-1920	P-GEAD-1921	P-GEAD-1922	P-GEAD-1923	P-GEAD-1924
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid labeling protocol	nucleic acid hybridization to array protocol	array scanning and feature extraction protocol	normalization data transformation protocol
Protocol Description	Sagittal and Coronal sections of FF and FFPE mouse brain samples were prepared on the Xenium slide(10x Genomics) using a cryostat (CM1950, Leica). Fixation and tissue permeabilization were performed according to the manufacturer's protocol.	according to the manufacturer's protocol.	Gene panel : Xenium Mouse Brain GEX	according to the manufacturer's protocol.	Fluorescent probe hybridization and imaging were conducted using the Xenium Analyzer (10x Genomics).	On board analysis was perfomed by the xenium-1.1.0.2.
SDRF File	E-GEAD-633.sdrf.txt
Comment[Number of channel]	single-channel
Comment[Array Design REF]	A-GEAD-11
Comment[AEExperimentType]	transcription profiling by array
Comment[BioProject]	PRJDB16277
Comment[Last Update Date]	2023-10-10