Comment[GEAAccession]	E-GEAD-664
MAGE-TAB Version	1.1
Investigation Title	Characterization of EPS-producing human commensal bacteria S. salivarius.
Experiment Description	Cultivation of S. salivarius in MRS medium containing 15% sucrose or 15% glucose at 30 degreeC for 10 h under anaerobic condition
Experimental Design	growth condition design	stimulus or stress design
Experimental Factor Name	medium
Experimental Factor Type	medium
Person Last Name	Kimura
Person First Name	Ikuo
Person Affiliation	Graduate School of Biostudies, Kyoto University
Person Roles	submitter
Public Release Date	2024-12-23
Protocol Name	P-GEAD-2082	P-GEAD-2083	P-GEAD-2084	P-GEAD-2085	P-GEAD-2086
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	Cultivation of S. salivarius in MRS medium containing 15% sucrose or 15% glucose at 30 degreeC for 10 h under anaerobic condition	Total RNA was extracted using the NucleoSpin RNA kit (Takara Bio inc., Tokyo, Japan) and immediately used for analysis.	Libraries are prepared by fragmenting a cDNA sample and ligating specialized adapters to both fragment ends. These adapters contain the full complement of sequencing primer hybridization sites.	The cDNA libraries were sequenced with Illumina Hiseq2500 platform.This dataset has paired-end reads of length 150 bp.	The parameters were set as follows: minimum length fraction = 0.8 and minimum similarity fraction = 0.8. Expression values were established as transcripts per million reads (TPM).
SDRF File	E-GEAD-664.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB17137
Comment[Last Update Date]	2024-12-23