Comment[GEAAccession]	E-GEAD-670
MAGE-TAB Version	1.1
Investigation Title	Transcriptome of mbd10 and mapkinase
Experiment Description	Arabidopsis METHYL-CPG-BINDING DOMAIN 10 (MBD10) is a protein that is phosphorylated in response to the plant hormone abscisic acid. Group C MAP kinase is a candidate kinase that phosphorylates MBD10.We investigated the variation in downstream genes whose expression is regulated by MAP kinase and MBD10.
Experimental Design	genotype design
Experimental Factor Name	genotype
Experimental Factor Type	genotype
Person Last Name	katagiri
Person First Name	sotaro
Person Affiliation	Tokyo University of agriculture and technology
Person Roles	submitter
Public Release Date	2024-01-18
Protocol Name	P-GEAD-2114	P-GEAD-2115	P-GEAD-2116	P-GEAD-2117	P-GEAD-2118
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	Hole plants were harvested and grinded in liquid nitrogen	RNAeasy	cDNA	NextSeq 500	Mapping was performed using bowtie2 to the Arabidopsis transcript database (TAIR10_cdna). Each column in the idx file indicates \"ID, Length, mapped read-segments, unmapped read-segments.\" idxstats in samtools was used to obtain the number of reads per gene. Normalization and statistical analysis were conducted using edgeR (version 3.42.0).
SDRF File	E-GEAD-670.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB16700
Comment[Last Update Date]	2024-01-18