Comment[GEAAccession]	E-GEAD-685
MAGE-TAB Version	1.1
Investigation Title	Characterization of joint MDSCs
Experiment Description	The purpose of this project is to characterize the myeloid derived suppressor cells (MDSCs) in the inflamed joints of arthritic SKG mice. We compared the gene expression of Joint MDSCs with MDSCs from bone marrow or spleen.
Experimental Design	disease state design
Experimental Factor Name	tissue
Experimental Factor Type	tissue
Person Last Name	Fujikawa
Person First Name	Yoshikazu
Person Affiliation	Rheumatology and Clinical Immunology, Kobe University
Person Roles	submitter
Public Release Date	2024-02-16
Protocol Name	P-GEAD-2190	P-GEAD-2191	P-GEAD-2192	P-GEAD-2193	P-GEAD-2194	P-GEAD-2195
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid labeling protocol	nucleic acid hybridization to array protocol	array scanning and feature extraction protocol	normalization data transformation protocol
Protocol Description	BD FACSAria III (BD Biosciences) was used to sort CD11b+Gr1+ cells (MDSCs) from BM, spleen and joints of arthritic SKG mice.	Total RNA was extracted from cultured cells using Maxwell RSC simplyRNA Cells Kit (Promega).	Biotinylated cRNA were prepared using GeneChip 3'IVT PLUS Reagent Kit (Thermo Fisher Scientific) according to the standard manufacturer's protocol from 100 ng total RNA.	Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the GeneChip Fluidics Station 450.	GeneChips were scanned using the GeneChip Scanner 3000 7G.	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Expresson Console Software (ThermoFisher Scientific) according to the default analysis settings.
SDRF File	E-GEAD-685.sdrf.txt
Comment[Number of channel]	single-channel
Comment[Array Design REF]	A-AFFY-45
Comment[AEExperimentType]	microRNA profiling by array
Comment[BioProject]	PRJDB17446
Comment[Last Update Date]	2024-02-16