Comment[GEAAccession]	E-GEAD-733
MAGE-TAB Version	1.1
Investigation Title	p53-inducible lncRNA LOC644656 causes genotoxic stress-induced stem cell maldifferentiation and cancer chemoresistance
Experiment Description	Genotoxic stress-induced stem cell differentiation is a protective mechanism in response to cell death. Herein, LOC644656 was identified as a p53-induced lncRNA that accumulates in the nuclei of human embryonic stem cells (hESCs) following genotoxic stress-induced maldifferentiation. We performed multi-omics analyses and identified LOC644656 functions in hESCs and cancer cell lines.
Experimental Design	compound treatment design	genetic modification design	transposable element identification design	reference design	replicate design
Experimental Factor Name	chem_administration
Experimental Factor Type	chem_administration
Person Last Name	YAMAGATA	TANAKA
Person First Name	Kazuyuki	Tomoaki
Person Affiliation	Department of Molecular Diagnosis, Chiba University Graduate School of Medicine, Chiba University	
Person Roles	submitter	submitter
Public Release Date	2024-11-09
Protocol Name	P-GEAD-2509	P-GEAD-2510	P-GEAD-2511	P-GEAD-2512	P-GEAD-2513
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	The cells were trypsinized using TrypLE select (Thermo Fisher Scientific), neutralized by AK02N, and centrifugated	gel/PCR DNA purification system (Viogene)	Tagment DNA Enzyme (TDE1) and Tagment DNA Buffer (TD) buffer kit (Illumina, 15027865)	Illumina HiSeq 1500	bigwig
SDRF File	E-GEAD-733.sdrf.txt
Comment[AEExperimentType]	ATAC-seq
Comment[BioProject]	PRJDB17987
Comment[Last Update Date]	2025-02-05