Comment[GEAAccession]	E-GEAD-846
MAGE-TAB Version	1.1
Investigation Title	HIV-associated neurocognitive disorder markers isolated from neuroexosomes
Experiment Description	To identify HIV-associated neurocognitive disorder (HAND) biomarker, transcriptome analysis of miRNAs extracted from circulating neuroexosomes isolated from HAND patients (MND patients: n=2, HAD patients: n=2) and non-HAND controls (n=4) was performed using the GeneChip miRNA 4.0 Array (Affymetrix).
Experimental Design	case control design	validation by reverse transcription PCR design
Experimental Factor Name	affection_status
Experimental Factor Type	affection_status
Person Last Name	Ayako	Kotaoro
Person First Name	Sedohara	Arizono
Person Affiliation	Division of Infectious Diseases, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo	
Person Roles	submitter	submitter
Public Release Date	2025-01-21
PubMed ID	39821903
Protocol Name	P-GEAD-3214	P-GEAD-3215	P-GEAD-3216	P-GEAD-3217	P-GEAD-3218	P-GEAD-3219
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid labeling protocol	nucleic acid hybridization to array protocol	array scanning and feature extraction protocol	normalization data transformation protocol
Protocol Description	Total exosomes were extracted from plasma using ExoQuick (System Biosciences) according to the manufacture's protocol. To isolate neuroexosomes, immunoprecipitation was performed using anti-L1CAM antibody.	Exosomal miRNAs were extracted using the miRNeasy Mini Kit (QIAGEN) according to the manufacture's protocol. The miRNA concentration and quality were evaluated using Agilent Bioanalyzer 2100 system (Agilent Technologies).	As per Affymetrix instructions.	As per Affymetrix instructions.	Standard Affymetrix array scanning and MAS5 extraction.	Standard affymetrix protocols.
SDRF File	E-GEAD-846.sdrf.txt
Comment[Number of channel]	single-channel
Comment[Array Design REF]	A-MTAB-537
Comment[AEExperimentType]	microRNA profiling by array
Comment[BioProject]	PRJDB18348
Comment[Last Update Date]	2025-01-21