Comment[GEAAccession]	E-GEAD-880
MAGE-TAB Version	1.1
Investigation Title	Cdkn1a mediates mouse line- and fate-dependent cellular responses in Cx3cr1-Cre genetic tools
Experiment Description	Microglia, resident macrophages in the central nervous system (CNS), play pivotal roles in maintaining tissue homeostasis. Genetic tools have facilitated precise monitoring and manipulation of microglia, enabling in-depth analyses to uncover their biological functions. Among the tools for studying microglia, tamoxifen (TAM)-inducible Cx3cr1-Cre mouse line (Cx3cr1CreERT2) is the most widely used tool in which the CreERT2 cassette is knocked into the genomic locus of CX3C chemokine receptor 1.. In the present study, we comparatively characterized currently available two Cx3cr1CreERT2 mouse models.
Experimental Design	genetic modification design
Experimental Factor Name	manipulation	epigenome	replicate
Experimental Factor Type	manipulation	epigenome	replicate
Person Last Name	Kishi
Person First Name	Yusuke
Person Affiliation	Laboratory of Molecular Neurobiology  Institute for Quantitative Biosciences, The University of Tokyo
Person Roles	submitter
Public Release Date	2025-08-06
Protocol Name	P-GEAD-3390	P-GEAD-3391	P-GEAD-3392	P-GEAD-3393	P-GEAD-3394
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	FACS isolation	Tn5 tagmentation	Tn5 tagmentation	Nextseq2000	Count per million reads
SDRF File	E-GEAD-880.sdrf.txt
Comment[AEExperimentType]	ATAC-seq
Comment[BioProject]	PRJDB19577
Comment[Last Update Date]	2025-08-06